| Hour 1 |
| Get Up! Go Insane! - Stretch & Vern |
| My Fault (Pizza Mix) - Eminem |
| Wa-Elegy (Waluigi's Assist Trophy Song) - Bitfinity (Matthew Taranto) |
| Ukyo Kuonji (2020) - TV's Kyle |
| Ranma Soutome - great Luke Ski, The |
| Dumb Newsbreak w/ Wacky Ben |
| OKeh Laughing Record, The - OKeh Record Company |
| Casey at the Bat - DeWolf Hopper |
| Uncle Josh Buys An Automobile - Cal Stewart |
| (I Am) The Fly - Michael Peyser & The Swat Team |
| Human Fly - Cramps, The |
| Professor's Theme - Katsuo Ono |
| WAP (Wet Ass Poopies) - MC Lars feat. Ash Tell Em |
| Stewie Sings Lucid Dreams - Azerrz |
| Eternal Shopping Mall - Nuclear Bubble Wrap |
| Bald Like Caillou - Akeem Lawanson |
| Maybe I'm Immune - Late Late Show with James Corden, The |
| Konata's Theme (Okinawa Version) - Satoru Kosaki |
| Hour 2 |
| Spider Verses - Devo Spice feat. Creative Mind Frame (aka 1-Up), Insane Ian, Bonnie Gordon, TV's Kyle, LEX the Lexicon Artist & the great Luke Ski |
| I've No More Fucks to Give - Thomas Benjamin Wild, Esq. |
| Old Town Road (remix) - Lil Nas X feat. Billy Ray Cyrus |
| Comic Fan - Library Bards, The |
| Overworld Theme - Bug Too! OST |
| There is Nothin' Like a Wall - Randy Rainbow |
| Everyone's Here - Il Neige |
| Hey Spidey... Again! - Chris Wolvie |
| Opinion Rhapsody - Dustin Ahkuoi |
| Egg and I, The - Yoko Kanno |
| Impeachment 2019 - Rocky Mountain Mike |
| A Very Stable Genius - Randy Rainbow |
| Inferno - Mrs. Green Apple |
| Enemy is An Idiot But Our Team Members Are Major Idiots, The - Slayers OSTs |
| Hour 3 |
| Bad Guy - Billie Eilish |
| Dad Guy - Funk Turkey |
| Bat Guy - Michael William Hunter |
| Earth - Lil Dicky |
| Le Bal - Hans Reichel |
| Learn Kung Fu - Jeff Whitmire feat. Insane Ian |
| Whac-a-Mole - Jake Waters |
| Other Friends - Aivi & Surasshu (feat. Sarah Stiles, Zach Callison, DeeDee Magno Hall, Estelle & Michaela Dietz) |
| Art of Darkness - Stupendium, The |
| Wacked-Out Five |
| (Will You) Just Shut Up? - Tom Smith |
| WAPC (Wet-Ass Pussy Cat) - Devo Spice |
| Zen - X Ambassadors, K.flay & Grandson |
| We're All Doomed - Songify featuring “Weird Al” Yankovic |
| Call Me Mabel - Mabel Pines |
| Bonus Round, The |
| You Want Sum - Nightsicles, The |
| Florida Man - Ross Childs |